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Datafile metadata | How to use this sheet Details on how to use this sheet and what the cells mean can be found by visiting: | ||||||||
Data File Title: | BR06 samples | ||||||||
Data File Description: | Samples taken in the 5L bioreactor BR06 in the culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C. Protein induction promoted when biomass reached 30 g cdw/L and at 29°C for 38 h | ||||||||
Project SEEK ID: | https://ibisbahub.eu/projects/88 | ||||||||
Master metadata sheet guide | |||||||||
Associated Assay | |||||||||
Existing Assay SEEK ID: | https://ibisbahub.eu/assays/913 | ||||||||
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New Assay metadata | |||||||||
Study SEEK ID: | |||||||||
Assay Title: | |||||||||
Assay Description: | |||||||||
Assay type: | Experimental_assay_type | Powered by: | |||||||
Technology type: | Technology_type | ||||||||
SOP SEEK ID: | |||||||||
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Sample ID | Sample volume | Date time | CDW Eppendorf | CDW Filter | OD 600nm, 10mm | Glucose YSI | Glucose HPLC | Aetate HPLC | Citrate HPLC | Nanobody densitometry | Nanobody nanodrop | Glucose feed |
M0 | 12.884 | 5.17 | 305 | |||||||||
M1 | 16 | 2019-02-06T9:15:0+0000 | 0.20833333333335005 | 0.13432098765431277 | 0.52 | 11.26 | 12.332 | 0.321 | 5.174 | 305 | ||
M2 | 15 | 2019-02-06T10:0:0+0000 | 0.43452380952388064 | 0.21766666666662715 | 0.9199999999999999 | 9.87 | 11.965 | 0.388 | 5.176 | 305 | ||
M3 | 16 | 2019-02-06T10:40:0+0000 | 0.6500000000000554 | 0.4400000000001292 | 1.6600000000000001 | 10.5 | 11.421 | 0.491 | 5.221 | 305 | ||
M4 | 14 | 2019-02-06T11:20:0+0000 | 1.132142857142899 | 0.7629999999999804 | 2.665 | 10.2 | 10.099 | 0.594 | 5.066 | 305 | ||
M5 | 11 | 2019-02-06T12:0:0+0000 | 2.057142857142853 | 1.3462500000001043 | 4.6000000000000005 | 8.02 | 8.617 | 0.655 | 5.091 | 305 | ||
M6 | 10 | 2019-02-06T12:50:0+0000 | 3.6714285714285952 | 2.622500000000194 | 8.49 | 5.5600000000000005 | 5.752 | 0.709 | 5.258 | 305 | ||
M7 | 11.5 | 2019-02-06T13:47:0+0000 | 6.849999999999992 | 5.646666666666651 | 17.9 | 0.069 | 0.352 | 0.559 | 5.278 | 305 | ||
M8 | 12 | 2019-02-06T14:48:0+0000 | 10.064285714285749 | 9.142499999999831 | 25.9 | 0.009 | 0.292 | 5.018 | 305 | |||
M9 | 12 | 2019-02-06T15:30:0+0000 | 12.721428571428568 | 11.595000000000244 | 34 | 0.013 | 4.824 | 305 | ||||
M10 | 8 | 2019-02-06T16:45:0+0000 | 20.01428571428567 | 19.909999999999428 | 50.300000000000004 | 0.014 | 4.496 | 305 | ||||
M11 | 6 | 2019-02-06T17:35:0+0000 | 25.85714285714287 | 25.476666666666592 | 73 | 0.007 | 4.296 | 305 | ||||
M12 | 65 | 2019-02-06T18:2:0+0000 | 30.889285714285624 | 29.264999999999652 | 0 | 0 | 4.126 | 0.21047833636839666 | 0.13496013290417547 | 305 | ||
M13 | 57 | 2019-02-06T19:19:0+0000 | 31.20357142857141 | 29.09999999999968 | 76.4 | 0 | 3.95 | 0.11251657069740272 | 0.6584235328856378 | 305 | ||
M14 | 57.5 | 2019-02-06T20:22:0+0000 | 31.77857142857137 | 30.76874999999979 | 73 | 0.008 | 3.845 | 0.17385560101285347 | 0.5840145330181112 | 305 | ||
M15 | 9 | 2019-02-06T21:15:0+0000 | 32.485714285714266 | 30.98999999999963 | 82.8 | 0.012 | 3.744 | 305 | ||||
M16 | 39 | 2019-02-06T22:10:0+0000 | 32.95357142857146 | 30.94000000000019 | 82.8 | 0.012 | 3.688 | 0 | 0.5394450773903413 | 305 | ||
M17 | 35 | 2019-02-06T23:25:0+0000 | 33.732142857142826 | 32.233333333333306 | 87.4 | 0 | 3.618 | 0.2686523841377872 | 1.185275769937326 | 305 | ||
M18 | 56 | 2019-02-07T1:0:0+0000 | 34.17499999999994 | 34.440000000000026 | 86.6 | 0 | 3.523 | 0.3061500249712766 | 1.4351893557719924 | 305 | ||
M19 | 30 | 2019-02-07T3:0:0+0000 | 35.25357142857146 | 34.2150000000001 | 90.8 | 0 | 3.398 | 305 | ||||
M20 | 50 | 2019-02-07T7:20:0+0000 | 36.64642857142852 | 33.69499999999981 | 96.6 | 0 | 3.113 | 0.590821707824876 | 1.5522541454472574 | 305 | ||
M21 | 7 | 2019-02-07T9:0:0+0000 | 37.48928571428572 | 37.27500000000017 | 96 | 0.029 | 2.995 | 305 | ||||
M22 | 10 | 2019-02-07T11:30:0+0000 | 38.16071428571433 | 38.21500000000011 | 94.6 | 0 | 2.909 | 305 | ||||
M23 | 55 | 2019-02-07T13:10:0+0000 | 38.47500000000004 | 37.10999999999976 | 93.99999999999999 | 0.024 | 2.817 | 0.48860416004472534 | 0.8931305046246418 | 305 | ||
M24 | 42 | 2019-02-07T16:2:0+0000 | 37.925000000000004 | 37.84833333333326 | 94.80000000000001 | 0 | 2.623 | 1.188016637980217 | 1.7257911621528828 | 305 | ||
M25 | 56 | 2019-02-07T18:58:0+0000 | 38.521428571428594 | 36.739999999999554 | 97.6 | 0.005 | 2.304 | 1.4162752246428327 | 3.7996681616488357 | 305 | ||
M26 | 35.5 | 2019-02-07T22:0:0+0000 | 38.84285714285721 | 37.44499999999995 | 100.5 | 0.014 | 1.956 | 1.2316253611930068 | 2.560110352533859 | 305 | ||
M27 | 32 | 2019-02-08T0:30:0+0000 | 38.4107142857143 | 38.645000000000266 | 97.5 | 0.017 | 1.548 | 1.455113612414852 | 3.721836724025184 | 305 | ||
M28 | 138 | 2019-02-08T7:47:0+0000 | 38.228571428571435 | 38.80999999999979 | 99 | 0 | 0.689 | 1.418290556200339 | 4.360247639692193 | 305 | ||