Submitter: Susana Alonso Villela
Investigation: Effect of the induction temperature in nanobody...
Assays: Fed-batch culture of WK6 strain, without induction - BR01
Snapshots: No snapshots
5L bioreactor culture of reference strain E. coli WK6. Protein induction promoter when biomass reached 25 g cdw/L and at 29°C for 28 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli NbF12-10. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 37 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 32°C for 35 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 30 g cdw/L and at 29°C for 38 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 37°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 33°C for 6 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
5L bioreactor culture of recombinant strain E. coli CH10-12. Protein induction promoter when biomass reached 25 g cdw/L and at 30°C for 10 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BioPAT MFCS data for BR03 - part 1, BioPAT MFCS data for BR03 - part 2, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BioPAT MFCS data for BR02 - part 1, BioPAT MFCS data for BR02 - part 2, Gas analysis data for BR02 and BR03
Snapshots: No snapshots
5L bioreactor culture of strain E. coli WK6 in a batch/fed-batch strategy at 37°C. Characterization of biomass growth and oxygen transfer
Submitter: Susana Alonso Villela
Assay type: Cultivation Experiment
Technology type: Fed-batch
Investigation: Effect of the induction temperature in nanobody...
Organisms: Escherichia coli
SOPs: No SOPs
Data files: BR01 samples, BioPAT MFCS data for BR01 - part 1, BioPAT MFCS data for BR01 - part 2, Data analysis of the BR01, Gas analysis data for BR01
Snapshots: No snapshots
Samples taken in the 5L bioreactor BR01 in the culture of E. coli WK6 in batch and fed-batch strategy
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
Abstract (Expand)
Authors: Susana María Alonso Villela, Hazar Ghezal-Kraïem, Balkiss Bouhaouala-Zahar, Carine Bideaux, César Arturo Aceves Lara, Luc Fillaudeau
Date Published: 1st Feb 2021
Publication Type: Journal
DOI: 10.1007/s00253-021-11093-5
Citation: Appl Microbiol Biotechnol 105(3):1017-1030