Fed-batch culture of CH10-12 strain, induction at 28°C - BR02
5L bioreactor culture of recombinant strain E. coli CH10-12 in a batch/fed-batch strategy at 37°C (BR02). Protein induction promoted when biomass reached 25 g cdw/L and at 28°C for 12 h
SEEK ID: https://ibisbahub.eu/assays/909
Experimental assay
Projects: Scorpion antivenom nanobody production in Escherichia coli (Recombinant ...
Investigation: Effect of the induction temperature in nanobody producing E. coli strains
Study: Recombinant nanobody CH10-12 production at different induction temperatures and duration
Assay position: 1
Assay type: Cultivation Experiment
Technology type: Fed-batch
Organisms: Escherichia coli
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Created: 18th Apr 2025 at 08:16
Last updated: 28th Apr 2025 at 14:30
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https://orcid.org/0000-0001-8290-1635 
Scorpionism is a serious public health problem in tropical areas, especially in Africa, southern India, the Middle East, and Latin America. There are about 12 species of scorpions that are dangerous to humans and all belong to the Buthidae family. In particular, venom on scorpion Androctonus australis hector is particularly toxic to humans. Serotherapy uses antibodies or fragments of antibodies to target the neurotoxins of the scorpion venom. Nanobodies, camelid-derived antibodies, are more ...
Programme: This Project is not associated with a Programme
Public web page: Not specified
Start date: 6th Feb 2017
In the biopharmaceutical industry, nanobodies show promising properties for its application in serotherapy for serums targeting the most toxic components of scorpion venom. Nevertheless, the limited number of scientific publications on the production of nanobodies for antivenom productions poses a challenge. The reported yield is low (< 4 mg/L), biomass production is not reported, and the production in high cell density cultures have not been studied. Production conditions (temperature of ...
Submitter: Susana Alonso Villela
Studies: Characterization of E. coli WK6 strain under aerobic conditions, Characterization of E. coli WK6 strain under induction conditions, Recombinant nanobody CH10-12 and NbF12-10 production at low induction te..., Recombinant nanobody CH10-12 production at different induction temperatu...
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C - BR02, Fed-batch culture of CH10-12 strain, induction at 29°C - BR06, Fed-batch culture of CH10-12 strain, induction at 30°C - BR03, Fed-batch culture of CH10-12 strain, induction at 32°C - BR07, Fed-batch culture of CH10-12 strain, induction at 33°C - BR04, Fed-batch culture of CH10-12 strain, induction at 37°C - BR05, Fed-batch culture of NbF12-10 strain, induction at 29°C - BR08, Fed-batch culture of WK6 strain, induction at 29°C - BR09, Fed-batch culture of WK6 strain, without induction - BR01
Submitter: Susana Alonso Villela
Investigation: Effect of the induction temperature in nanobody...
Assays: Fed-batch culture of CH10-12 strain, induction at 28°C - BR02, Fed-batch culture of CH10-12 strain, induction at 30°C - BR03, Fed-batch culture of CH10-12 strain, induction at 33°C - BR04, Fed-batch culture of CH10-12 strain, induction at 37°C - BR05
Gas analyzer (INNOVA 1313, Lumasense Technologies) Channel 3: gas out - BR02 Channel 4: gas out - BR03 Channel 6: gas in (reference) Sampling time: 60 s Flushig time: 45 s Sampling time: 15 s
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h
5L culture of CH10-12, 37°C batch/fed-batch culture with induction at 28°C for 12h
Procedure followed for the data treatment and analysis of 5L bioreactor cultures
Creator: Susana Alonso Villela
Submitter: Susana Alonso Villela
